In vitro clonal propagation of vulnerable medicinal plant , Saraca asoca ( Roxb . ) De Wilde

In vitro clonal propagation of Ashoka, Saraca asoca (Roxb.) De Wilde plant was achieved using shoot tip, nodal and internodal explants. The explants were cultured on MS medium supplemented with different concentrations (0.5-2.0mg/l) of Benzyl amino purine (BAP), Kinetin (Kn) and 2, 4-Dichlorophenoxy acetic acid (2,4-D). The synergetic effect of BAP (0.5mg/ l) induced a mean of 11.71±0.53 adventitious shoots from the nodal explants. The frequency of shoot organogenesis was highest (82%) in nodal explants treated with 0.5mg/l of BAP and callus were formed more on 2, 4-D. The micro-shoots rooted well on MS medium supplemented with 4.0mg/l of IBA. Hardened regenerants (40%) were acclimatized to the soil.